Twenty-six isolates of Colletotrichum kahawae, the causal agent of coffee berry disease, from coffee in Africa, and 25 isolates, mostly of Colletotrichum gloeosporioides, from coffee and other tropical perennial crops, were examined for the ability to metabolize citrate and tartrate and their molecular genetic variability was assessed using restriction fragment length polymorphisms (RFLP) and variable number tandem repeats (VNTR). Twenty-four isolates of C. kahawae were also assessed using amplified fragment length polymorphisms (AFLP). Vegetative compatibility within a collection of nine isolates, including two of C. gloeosporioides was also assessed. All isolates of C. kahawae from across Africa failed to metabolize citrate or tartrate, but all other isolates metabolized one or both. Colletotrichum kahawae isolates also showed minimal variability using the molecular techniques with two isolates from Cameroon showing slightly different banding patterns in RFLP analysis. All other isolates had variable VNTR and RFLP banding patterns. AFLP analysis failed to detect variability within 12 isolates from Kenya, but did detect differences between isolates from other countries. Five isolates from Kenya were vegetatively compatible but differed from two from Cameroon and from two C. gloeosporioides isolates. Results demonstrate some geographic variability within C. kahawae isolates, although this is small, probably due to the relatively young age of C. kahawae populations. The biochemical and molecular techniques used showed clear differences from other Colletotrichum isolates, and can be used to distinguish the species. Lack of citrate and tartrate metabolism provides a readily applicable diagnostic method.
View Comments The Broadway.com staff is nuts about Culturalist, the website that lets you choose and rank your own top 10 lists. Every week, we’re challenging you with a new Broadway-themed topic to rank—we’ll announce the most popular choices on the new episode of The Broadway.com Show every Wednesday.Last week, we asked you to name the Broadway star you were rooting for on Tony nomination morning. The results are in, and fans picked The King and I star Kelli O’Hara! Your good vibes worked, and O’Hara nabbed her sixth nomination on April 28. This week, Mother’s Day is just around the corner, so we want to know: Who is the ultimate mom in a Broadway musial? We narrowed down the choices to 25 of our favorites. Broadway.com Features Editor Lindsay Champion posted her list of top 10 picks here!STEP 1—SELECT: Visit Culturalist to see all of your options. Highlight your 10 favorites and click the “continue” button.STEP 2—RANK: Reorder your 10 choices by dragging them into the correct spot on your list. Click the “continue” button.STEP 3—PREVIEW: You will now see your complete top 10 list. If you like it, click the “publish” button. (If you don’t have a Culturalist, you will be asked to create one at this point.)Once your list is published, you can see the overall rankings of everyone on the aggregate list.Pick your favorites, then tune in for the results on the next episode of The Broadway.com Show!
By Julieta Pelcastre/Diálogo August 03, 2018 The Guatemalan Navy, with the support of the U.S. Marine Corpsy, trained a naval component in the Advanced Marine Course in Puerto Barrios, Izabal, throughout May 2018. The objective of the course was to improve the response capabilities of the Guatemalan Marines to conduct operations in various environments. “We thank the U.S. government for contributing to the training of our marines,” Captain Erick Roberto Orellana, second commander of the Guatemalan Marine Brigade, told Diálogo. “It helped us strengthen our skills and capabilities to fulfill our amphibious and riverine missions in coastal areas.” Soldiers of steel The training consisted of high-performance tactical phases in areas such as urban operations, water combat, use of support weapons, as well as incursion and amphibious landing techniques in waters 1 kilometer off the coast. “Soldiers have to bear the weight of their own body, plus the weight of all the equipment and weapons,” said Capt. Orellana. “It’s hard, because it’s twice the effort to get to shore without sinking or drowning and without being noticed.” With the support of U.S. service members, Guatemalan troops learned to take position in a war ship. They received specific combat training to respond to an ambush or enemy fire, and trained with modern physical conditioning techniques. U.S. service members also provided operational advice to naval and land officers of the Guatemalan Marine Brigade. The Advanced Marine Course demands physical and mental strength, and not every candidate is able to complete the course and graduate. “Out of 80 students accepted, only 25 passed the course,” said Capt. Orellana. “We don’t want supermen; we seek to train elements that will contribute to maintaining the defense of sovereignty and deliver maximum results in the fight against national and international criminal organizations.” Integrated cooperation The Guatemalan Marine Brigade contributes to the development of joint and combined military operations against any threat to the general public. According to the Guatemalan Ministry of Defense, the unit seeks to be the best-trained strategic brigade in the country to conduct operations in coastal, riverine, amphibious, and special environments. Collaboration with the armed forces of the Western Hemisphere allows Guatemalan Armed Forces to improve their sea, air, and land defense. The U.S. Department of State, in its 2018 International Narcotics Control Strategy Report, estimates that about 1,400 tons of cocaine were smuggled through the country in 2017, most was destined for the U.S market. “Integrated cooperation with the U.S., Colombian, Mexican, and Chilean governments allows us to be trained, improve our own doctrine, and apply it in our fields to attain the best outcome in an operation, and make completion of tasks more dynamic,” Navy Lieutenant Eduardo Antonio Carmona, chief of the Naval Training Center of the Guatemalan Marine Forces, told Diálogo. “We have a more technical training with the U.S. military, while we focus more on the military’s own capabilities when we work with Latin American forces. “Sometimes equipment or resources are inadequate, but marines are trained to complete assigned tasks with available resources. Marines know and trust they can fulfill their mission quickly and effectively, no matter how good their weapons or boots are.” The Guatemalan Marine Brigade has receives tactical and physical training from the United States since 2013. According to Lt. Carmona, a total of 540 marines have been trained by way of the advanced course. “Thanks to this cooperation we seized a huge amount of drugs in the last three years,” Capt. Orellana concluded. U.S. Marine Corps amphibious troops will offer two more courses in 2018.
England midfielder Frank Lampard has retired from international football after winning 106 caps.The 36-year-old was released by Chelsea in May after 13 years at the London club and he signed for New York City before agreeing to move to Manchester City on a six-month loan deal.Lampard scored 29 goals for England and captained the team in their last group match against Costa Rica at this year’s World Cup in Brazil.”I have taken the decision to retire from international football,” he said in a statement on Tuesday.”It has been a very tough decision for me to make which is why I have given it so much thought since the World Cup. I have always been exceptionally proud and honoured to represent my country and have to say looking back I have enjoyed every minute of wearing the England shirt.”Lampard said it was time put his family first given that he was soon to be playing in the United States. “Also, to concentrate on how I can perform consistently to the best of my abilities over my next few years in club football,” he added.”It is now the time to move forward and I feel very confident that with Roy Hodgson in charge, the young players that we have coming through, and the changes that are being made throughout the development of the youth system in this country, that we will have success in the future and a team that this country deserves.”England captain Steven Gerrard ended his long international career last month.Lampard made his international debut against Belgium in 1999, going on to play at three World Cups and two European Championships.He make headlines at the 2010 World Cup in South Africa when he had a goal wrongly disallowed in a second-round clash with Germany, a match England went on to lose 4-1.
Authorities later discovered that Montalvo’s phone had been turned off and that her car was abandoned about 8-miles from her son’s grandparent’s home.After conducting an extensive search of the father and grandparent’s home Friday, authorities discovered Montalvo’s remains.Montalvo’s ex-husband Christopher Otero-Rivera and his father Angel Rivera were arrested Friday on unrelated charges, however, according to court records both men are now facing a premeditated murder charge and are being held without bond. The Osceola County Sheriff’s department is reporting that investigators have found the remains of a mother who was reported missing last week after she failed to pick up her son from school.35-year-old Nicole Montalvo went missing on October 21st after she dropped her 8-year-old son off to his father and grandparent’s home. Authorities were then notified about her disappearance Tuesday afternoon when she failed to pick up her son from school and no one was able to get in touch with her.Florida mother reported missing after failing to pick up son from school
A possible case of coronavirus here in South Florida recently affected emergency operations at a hospital, according to reports.Workers at Memorial Regional Hospital in Broward allegedly told at least one fire-rescue department not to bring anyone to their emergency room.Dr. Bindu Mayi, an infectious disease specialist and professor of microbiology at Nova Southeastern University, says she had heard about the potential Hollywood case from medical colleagues.“If there is a local case, it is something we [the public] should all be made aware of,” Mayi says. “The absence of information becomes conjecture and that’s a dicey thing. If there is a local case, people who are immune-suppressed may need to be more discerning about not being in large crowds or gathering where don’t know who has been exposed to what.”Memorial Regional Hospital officials would not comment on the status of the woman who arrived last Friday afternoon in their emergency room. Hospital spokeswoman Kerting Baldwin explains, “There are no confirmed cases of coronavirus at Memorial.”Hollywood Fire Rescue was informed of the situation in the emergency room. “At one point we offered to wear masks, but that was overkill because the patient suspected of coronavirus was already in isolation,” says Patrick Moore, division chief of operations at Hollywood Fire Rescue.Amanda Conwell, a spokesperson for the Pembroke Pines Police Department, says her staff was told for a brief time Friday evening not to transport anyone to Memorial until a proper quarantine was established. She adds that the action was based on information they had and did not indicate that there was a confirmed case within the county.Meanwhile, the Florida Department of Health will not comment on the possible case.State health officials explain they are concerned about creating panic or compromising patient privacy by disclosing too much information. They may use their website to inform the public about pending cases and results.“We’ve kicked it around and bounced it off leadership and we hope to have something soon,” says Brad Dalton, a spokesman for the Florida Department of Health. “You don’t want to scare people, and you want to make sure when information gets out there it’s what does the public the most good.”The number of confirmed cases in the U.S. stood at 12 on Wednesday.Tests were pending on 76 possible coronavirus cases across the country as of Wednesday. The CDC does not indicate where in the United States those tests have been conducted, although at least some of them have come from Florida.A week ago, Gov. Ron DeSantis said during a visit to Boca Raton that all tests for coronavirus in our state were sent to the CDC in Atlanta and had come back negative.South Florida hospitals say that staff from the health department have met with them in order to ensure they are properly screening and preparing for a possible coronavirus case here.Local doctors also believe it is only a matter of time before coronavirus reaches Florida.“We know we are at high risk with international travelers coming to our area,” says Dr. Ivan Gonzalez, a pediatric infectious disease doctor at Jackson Memorial and UM Health. “A lot of this is going to rely on self-reporting — awareness and education are key.”A quarantine station at Miami International Airport that is staffed by the CDC is screening international passengers flying into the region.Florida’s Department of Health says it is unclear when and where tests kits will be widely available in Florida.The virus originated in Wuhan, China. It has spread to 28 countries. There are more than 24,000 confirmed cases in China alone.Last week, the U.S. Department of Health and Human Services declared coronavirus a public health emergency, imposing a mandatory quarantine for any citizen who had traveled to China’s Hubei province during the previous two weeks.In addition, planes carrying U.S. citizens who have recently been in China are only allowed to land at one of 11 designated airports, none of which are in Florida. As of Wednesday, more than 500 people have died as a result of the virus.