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Variability of Colletotrichum kahawae in relation to other Colletotrichum species from tropical perennial crops and the development of diagnostic techniques

first_imgTwenty-six isolates of Colletotrichum kahawae, the causal agent of coffee berry disease, from coffee in Africa, and 25 isolates, mostly of Colletotrichum gloeosporioides, from coffee and other tropical perennial crops, were examined for the ability to metabolize citrate and tartrate and their molecular genetic variability was assessed using restriction fragment length polymorphisms (RFLP) and variable number tandem repeats (VNTR). Twenty-four isolates of C. kahawae were also assessed using amplified fragment length polymorphisms (AFLP). Vegetative compatibility within a collection of nine isolates, including two of C. gloeosporioides was also assessed. All isolates of C. kahawae from across Africa failed to metabolize citrate or tartrate, but all other isolates metabolized one or both. Colletotrichum kahawae isolates also showed minimal variability using the molecular techniques with two isolates from Cameroon showing slightly different banding patterns in RFLP analysis. All other isolates had variable VNTR and RFLP banding patterns. AFLP analysis failed to detect variability within 12 isolates from Kenya, but did detect differences between isolates from other countries. Five isolates from Kenya were vegetatively compatible but differed from two from Cameroon and from two C. gloeosporioides isolates. Results demonstrate some geographic variability within C. kahawae isolates, although this is small, probably due to the relatively young age of C. kahawae populations. The biochemical and molecular techniques used showed clear differences from other Colletotrichum isolates, and can be used to distinguish the species. Lack of citrate and tartrate metabolism provides a readily applicable diagnostic method.last_img read more

Stevie Wonder, John Scofield Tapped For Miles Davis Tribute Album

first_imgFew musicians are as symbolic of an entire genre of music as Miles Davis is to jazz. The two are literally inseparable, perhaps driving Don Cheadle to write, direct, and star in the Miles Ahead biopic (watch the trailer here). Another tribute to Davis is on the way, as jazz pianist Robert Glasper has organized an album dedicated to the iconic trumpeter.Titled Everything’s Beautiful, Glasper has recruited a who’s who of musicians for the Miles Davis tribute project. Among those involved: Stevie Wonder, John Scofield, Erykah Badu, Hiatus Kaiyote, Bilal, Illa J and so many more. The album is due out May 27th via Sony Records, and pre-orders can be placed here.Check out the tracklisting below:Everything’s Beautiful Tracklisting: 1. “Talking Shit” 2. “Ghetto Walkin” featuring Bilal 3. “They Can’t Hold Me Down” featuring Illa J 4. “Maiysha (So Long)” featuring Erykah Badu 5. “Violets” featuring Phonte 6. “Little Church” featuring Hiatus Kaiyote 7. “Silence Is The Way” featuring Laura Mvula 8. “Song For Selim” featuring KING 9. “Milestones” featuring Georgia Ann Muldrow 10. “I’m Leaving You” featuring John Scofield and Ledisi 11. “Right On Brotha” featuring Stevie Wonderlast_img read more

Day of Long Knives as Maigari Battles Pinnick for the Soul…

first_imgDuro IkhazuagbeFinally, the battle for the soul of Nigerian football will be decided Thursday in Katsina as incumbent Amaju Melvin Pinnick guns for a second term in office as president of the Nigeria Football Federation (NFF). Others in the race include, immediate past president, Aminu Maigari, a former General Secretary, Taiwo Ogunjobi and a dark horse amateur club proprietor, Chinedu Okoye.Pinnick is aiming to break a jinx that has lasted over 50 years as none of the past presidents has ever succeeded in returning to office back-to-back. The former Delta FA chairman is banking on his achievements during his first term to swing victory in his favour. But football historians in the country know that winning the NFF presidency goes beyond performance in office. Extraneous factors bothering on the usual Nigerian ways of doing things take the lion share in who gets elected. Ordinarily, Pinnick’s performance in this first spell ending today is enough to get him an automatic ticket for an encore on the job. Apart from raising the profile of the federation with multi-billion Naira sponsorship partnerships, he has succeeded in putting NFF on the path to self-sustainability! Instead of the previous beggarly disposition of the NFF, now, Nigerians are beginning to see semblance of corporate governance in the running of the affairs of the Glass house. And only a second term can consolidate all the gains recorded in the past four yearsBut Maigari whose secondary education certificate is under scrutiny going into today’s polls at the Kabir Aliyu Maska Conference Hall in Katsina, is a time-tested gladiator of the Nigerian football politics.After high-wired conspiracy stopped him from getting re-elected shortly after the World Cup in Brazil where Super Eagles matched the country’s previous best achievement of a second-round finish, the Bauchi-born football administrator is banking on the structure he has been oiling since 2010 when he succeeded Sani Lulu Abdullahi to reclaim his mandate from Pinnick. He is also banking heavily on the previous block votes that come from the northern delegates who speak with one voice.However, Maigari’s permutation may have been punctured by the young Turks led by Shehu Dikko, an alley of Pinnick and Ibrahim Gusau who are both gunning to return to the board as both 2nd Vice President and Chairman of Chairmen respectively. Dikko also doubles as chairman of the League Management Company (LMC). They both know their return to these two plum positions are tied to Pinnick’s fate today. Gusau has oil and gas magnate, Ifeanyi Ubah to contend with for the chairman of chairmen position.As at last night, wheeling and dealings amongst the combatants stretched late into the night with the various camps crossing the ‘ts’ and dotting the ‘is’.For the position of the 1st Vice President, two candidates from the South-west are at each others throats. Incumbent Lagos FA Chairman, Seyi Akinwunmi is aiming for return while a former member of the Pinnick camp, Otunba Sunday Dele-Ajayi wants the same position. Unless something gives in the last minute horse-trading that dove-tailed into today’s polls, the Lagos FA boss is as good as returned to the position.An interesting scenario is also expected to play out in which candidate represents the South-east in the new board. While Chairman of Enyimba, Felix Anyasi-Agwu is plotting his return, Vice-chairman of Imo FA Emmanuel Ochiagha is insisting it is the turn of Imo to represent the zone on the NFF board. Another candidate from Abia, Emeka Inyama is likely to step down.Share this:FacebookRedditTwitterPrintPinterestEmailWhatsAppSkypeLinkedInTumblrPocketTelegramlast_img read more